This book on Instrumental Methods of Analysis provides a clear, systematic, and student-friendly approach to understanding modern analytical techniques used in pharmaceutical and chemical analysis. The content is carefully structured to simplify complex concepts through well-organized explanations, illustrations, flow charts, and practical examples, making it easier for learners to grasp both theoretical and instrumental aspects.
The book covers a wide range of analytical methods including UV-Visible spectroscopy, fluorimetry, IR spectroscopy, flame photometry, atomic absorption spectroscopy, and nepheloturbidometry. It also offers comprehensive coverage of separation techniques such as chromatography and electrophoresis, including thin layer chromatography, paper chromatography, gas chromatography, HPLC, ion-exchange, gel, and affinity chromatography. Each topic is presented with its principle, instrumentation, and applications to provide a complete understanding of the techniques.
To support effective learning and examination preparation, each section includes a set of long answer and short answer questions. With its lucid language and structured presentation, this book serves as a valuable resource for students and teachers, helping to build strong analytical skills and a solid foundation in instrumental analysis.
1. UV - Visible Spectrosocpy
1.1 Electronic Transitions
1.2 Chromophores
1.3 Auxochromes
1.4 Spectral Shifts
1.5 Solvent Effect on Absorption Spectra
1.6 Beer And Lambert’s Law
1.7 Derivation And Deviations
1.8 Instrumentation
1.9 Sources of Radiation
1.10 Wavelength Selectors
1.11 Sample Cells
1.12 Detectors
1.13 Photo Tube
1.14 Photomultiplier Tube
1.15 Photo Voltaic Cell
1.16 Silicon Photodiode
1.17 Applications
1.18 Spectrophotometric titrations
1.19 Single component and multi component analysis
2. Fluorimetry
2.1 Theory
2.2 Concepts of Singlet, Doublet and Triplet Electronic States
2.3 Internal and External Conversions
2.4 Factors Affecting Fluorescence
2.5 Quenching
2.6 Instrumentation and Applications
3. IR Spectrosocpy
3.1 Introduction
3.2 Fundamental modes of vibrations in poly atomic molecules
3.3 Sample handling
3.4 Factors affecting vibrations
3.5 Instrumentation
3.6 Sources of radiation
3.7 Wavelength selectors
3.8 Detectors - Golay cell
3.9 Bolometer
3.10 Thermocouple
3.11 Thermister
3.12 Pyroelectric detector and applications
4. Flame Photometry
4.1 Flame Photometry
4.2 Principle
4.3 Interferences
4.4 Instrumentation and Applications
5. Atomic Absorption Spectrosocpy
5.1 Principle
5.2 Interferences
5.3 Instrumentation and Applications
6. Nephloturbidometry
6.1 Principle
6.2 Instrumentation
6.3 Applications
7. Introduction To Chromatography
7.1 Introduction
7.2 Theories of Chromatography
7.3 Principle
7.4 Classification of Chromatography
8. Adsorption Chromatography
8.1 Introduction
8.2 Principle
8.3 Methodology
8.4 Types of adsorption chromatography
8.5 Limitations ofadsorption chromatography
8.6 Applications
9. Thin Layer Chromatography
9.1 Introduction
9.2 Principle
9.3 Methodology
9.4 Rf values
9.5 Advantages
9.6 Disadvantages and Applications
10. Paper Chromatogrraphy
10.1 Introduction
10.2 Methodology
10.3 Development Techniques
10.4 Advantages, Disadvantages and Applications
11. Electrophoresis
11.1 Introduction
11.2 Factors affecting electrophoretic mobility
11.3 Techniques of paper
11.4 Gel
11.5 Capillary electrophoresis
11.6 Applications
12. Gas Chromatography
12.1 Introduction
12.2 Theory
12.3 Instrumentation
12.4 Derivatization
12.5 Temperature Programming
12.6 Advantages, Disadvantages And Applications
13. High Performace Liquid Chromatography (HPLC)
13.1 Introduction
13.2 Theory
13.3 Instrumentation
13.4 Advantages and applications
14. Ion-Exchange Chromatography
14.1 Introduction
14.2 Classification
14.3 Ion exchange resins
14.4 Properties
14.5 Mechanism of ion exchange process
14.6 Factors affecting ion exchange
14.7 Methodology and applications
15. Gel Chromatography
15.1 Introduction
15.2 Theory
15.3 Instrumentation
15.4 Applications
16. Affinity Chromatography
16.1 Introduction
16.2 Theory
16.3 Instrumentation
16.4 Applications
A book as per the latest syllabus given by Pharmacy Council of India (PCI) New Delhi
for B.Pharm students of all Indian Universities
