A Textbook of Advanced Instrumentation Techniques

1. Nuclear Magnetic Resonance Spectroscopy
1. Introduction
2. Principles of 1HNMR and 13C NMR
3. Chemical shift
4. Factors affecting chemical shift.
5. Coupling constant
6. Spin - spin coupling, relaxation.
7. Instrumentation
8. 13C NMR
9. Nuclear Overhauser Effect
10. Applications

2. Mass Spectrometry
1. Introduction and History
2. Principle
3. Fragmentation
4. Ionization techniques : Electron impact, chemical ionization. MALDI, FAB.
5. Analyzers : Time of flight and Quadruple
6. Instrumentation
7. Applications

3. Thermal Methods of Analysis
1. Introduction
2. Thermo gravimetric Analysis (TGA)
a. Principle
b. Instrumentation
c. Applications
3. Differential Thermal Analysis (DTA)
a. Principle
b. Instrumentation
c. Applications
4. Differential Scanning Calorimetry (DSC)
a. Principle
b. Instrumentation
c. Applications

4. X-Ray Diffraction
1. Introduction
2. Origin of X-ray
3. Basic aspects of crystals
4. X-ray crystallography
5. Rotating crystal technique
6. Single crystal Diffraction
7. Powder diffraction
8. Structure elucidation
9. Applications

5. Calibration and Validation
1. Introduction
2. Calibration
a. As per ICH
b. As per USFD
3. Validation
a. As per ICH
b. As per USFDA
4. Applications

6. Calibration of Instruments
1. Electronic Balance
2. UV-Visible Spectrophotometer
3. IR Spectrophotometer
4. Fluorimeter
5. Flame Photometer
6. HPLC
7. GC

7. Radio Immune Assay
1. Introduction and Importance
2. Principle
3. Various Components
4. Different Methods
5. Limitations
6. Applications of Radio immune assay

8. Extraction Technique
1. General Principle of Solid Phase Extraction
2. Procedure of Solid Phase Extraction
3. General Principle of Liquid-Liquid Extraction
4. Procedure of Liquid- Liquid Extraction

9. Hyphenated Technique
1. Introduction to Hyphenated techniques.
2. LC-MS/MS.
3. GC-MS/MS.
4. HPTLC-MS.